Transactions on Additive Manufacturing Meets Medicine
Vol 3 No 1 (2021): Trans. AMMM
https://doi.org/10.18416/AMMM.2021.2109532

Scaffolds, Implants and Drug Delivery Systems, ID 532

Development of in vitro 3D brain models on laser fabricated scaffolds

Main Article Content

Ayman El-Tamer (Laser nanoFab GmbH), Andrea Deiwick  (Institute of Quantum Optics, Leibniz University Hannover, Hannover, Germany), Lothar Koch (Institute of Quantum Optics, Leibniz University Hannover, Hannover, Germany), Jordi Soriano (Universitat de Barcelona Institute of Complex Systems (UBICS), Barcelona, Spain and Departament de Física de la Matèria Condensada, Universitat de Barcelona, Barcelona, Spain), Ulf Hinze (Laser nanoFab GmbH and Institute of Quantum Optics, Leibniz University Hannover, Hannover, Germany), Boris Chichkov (Institute for Regenerative Medicine, Sechenov University, Moscow, Russia), Anastasia Koroleva (Institute of Quantum Optics, Leibniz University Hannover, Hannover, Germany and Institute for Regenerative Medicine, Sechenov University, Moscow, Russia)

Abstract

In this work, we present the design and implementation of a 3D scaffold platform that supports and promotes intricate neuronal network development. 3D scaffolds were produced by two-photon polymerization (2PP), a high-resolution 3D laser microstructuring technology, using the biocompatible and non-degradable photopolymer. Human iPSC-derived neuronal networks have developed and interconnected in a 3D environment shaped by vertically stacked scaffold layers. The iPSC-derived neuronal progenitor cells could develop into cortical projection neurons (CNPs) of all six layers, different types of inhibitory neurons and glia. The network showed strong neuronal spontaneous activity that combined individual and collective signaling events. Our results highlight advanced microstructured 3D scaffolds as a reliable platform for 3D in vitro modelling of neuronal functions.

Article Details

How to Cite

El-Tamer, A., Deiwick , A. ., Koch, L., Soriano, J., Hinze, U., Chichkov, B., & Koroleva, A. (2021). Development of in vitro 3D brain models on laser fabricated scaffolds. Transactions on Additive Manufacturing Meets Medicine, 3(1), 532. https://doi.org/10.18416/AMMM.2021.2109532