Transactions on Additive Manufacturing Meets Medicine
Vol. 6 No. S1 (2024): Trans. AMMM Supplement
https://doi.org/10.18416/AMMM.2024.24091864

DFG Minisymposium Research Unit 5250: Mechanism-based characterisation and modelling of permanent and bioresorbable implants, ID 1864

In vitro cytocompatibility and first in vivo results of additively manufactured titanium implants with polymer multilayer coatings

Main Article Content

Ole Jung (University Medical Center Rostock), Cindy Reinholdt (1 Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Strempelstraße 13, 18057 Rostock, Germany), Mike Barbeck (1 Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Strempelstraße 13, 18057 Rostock, Germany), Anne Kathrin Jung (1 Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Strempelstraße 13, 18057 Rostock, Germany), Rumen Krastev (2 Reutlingen University, Reutlingen, Germany), Tonya Andreeva (2 Reutlingen University, Reutlingen, Germany), Anne Jahn (3 Laser Zentrum Hannover e.V., Hannover, Germany)

Copyright (c) 2024 Ole Jung, Cindy Reinholdt, Mike Barbeck, Anne Kathrin Jung, Rumen Krastev, Tonya Andreeva, Anne Jahn

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Introduction: Titanium and its alloys are extensively used materials in surgical fields such as oral surgery following trauma, tumors, or malformations due to their high biocompatibility, excellent bone integration, and favorable mechanical properties. Ensuring a stable healing process requires effective tissue interaction and osseointegration, where the surface structure and implant modifications play a crucial role in tissue regeneration. Surface modifications can influence wettability, cell proliferation, and growth.


Objectives: The aim of the DFG Research Group 5250 “Permanent and bioresorbable implants with customized functionality” is the development and validation of an innovative approach for the manufacturing, characterization and simulation-based design of additively manufactured implants for various surgical disciplines.


Material and methods: Additively manufactured titanium discs (Ti-6Al-4V ELI) with three different roughnesses and surface coatings (PAA/PAH, PAA/PSS, HA/Chi) underwent cytocompatibility testing to the standard protocol DIN EN ISO 10993-5:2009. Cell proliferation (BrdU-assay), viability (XTT-assay) and cytotoxicity (LDH-assay) were assessed indirectly by exposing L929 mouse fibroblasts and MC3T3 mouse preosteoblasts to extracts of the specimens. Surface coating and roughness compatibility with cell adhesion was evaluated directly by staining adherent cells on the test materials. Based on the in vitro tests, the favored implants were implanted subcutaneously in rats for 10 and 30 days according to the 3R principle. After euthanization, the samples were processed histologically in accordance with DIN EN ISO 10993-6:2017 to demonstrate the hisopathological and inflammatory processes.


Results: All extracts of the different surface coatings and roughnesses indicated no inhibitory effect on cell proliferation and cell viability nor a cytotoxic activity on the cells. Additionally, no restriction on cell attachment ability on the materials could be observed. Initial in vivo results showed promising outcomes compared to untreated titanium control.


Conclusion: Excellent cyto- and promising biocompatibility was demonstrated by all materials and coatings in accordance with the 3R principle.

Article Details

How to Cite

Jung, O., Reinholdt, C., Barbeck, M., Jung, A. K., Krastev, R., Andreeva, T., & Jahn, A. (2024). In vitro cytocompatibility and first in vivo results of additively manufactured titanium implants with polymer multilayer coatings . Transactions on Additive Manufacturing Meets Medicine, 6(S1), 1864. https://doi.org/10.18416/AMMM.2024.24091864