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During the development of a device for the injection of stem cells into a non-union, several components had to be considered. Focus was on handling, whereby the radiation exposure for the surgeon and the surgical personnel is to be reduced and the insertion of a depth limiter ensures proper injection of the stem cells into the non-union. Another part of major importance of the device is the cannula. Until now it is not clear if the injection process of stem cells through a cannula influences the vitality of the cells. To investigate the vitality after injection, a test setup was developed in which a cell suspension can be injected with a constant volume flow.